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To disambiguate the potency ranking of covalent inhibitors, right here we explain a data-analytic process relying on two independent IC 50 values, determined at two various reaction moments.In the case of covalent inhibitors pursuing the two-step kinetic system Age I Age I EI, thé two IC 50 ideals by itself can be utilized to calculate both the inhibition constant ( K we ) as a gauge of presenting affinity and the inactivation rate constant ( k inact ) as a gauge of chemical reactivity.
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